LOCUS       sequence-96809-e         561 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..561
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      334..343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(510..529)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcaccatgtc tcctcccagc agctcggtca ccatctccag ctggtcggcc gtggagaagc
       61 tcccgccacc gccgtcgttg tctccccgaa gggagaaggg tgtgaccgca acgtaggagg
      121 gcgagcagag cccggagcgg cggctagggg acaggggcgg ggtgggcagc agctcgaatt
      181 tcttccagat atcctcgctg ggcgccgggg gctgcagctc gctctgctgc tgctgctggt
      241 agaagttctc ctcctcgtcg cagtagaaat acggctgcac cgagtcgtag tcgaggtcat
      301 agttcctgtt ggtgaagcta acgttgaggg ggcccatggt ggcgaattgg tattatcgtg
      361 tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg
      421 actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta
      481 ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt
      541 ccacaactat ccaactcaca a
//
