LOCUS       sequence-96811-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.06"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "246-54"
COMMENT     Sequence Label: T7-VEE-OKS-iM
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      562..571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taacattgta atcgcaagca gagtgttgag agaacggcta accggatcac catgtgcagc
       61 attcattgga gatgacaata tcgtgaaagg agtcaaatcg gacaaattaa tggcagacag
      121 gtgcgccacc tggttgaata tggaagtcaa gattatagat gctgtggtgg gcgagaaagc
      181 gccttatttc tgtggagggt ttattttgtg tgactccgtg accggcacag cgtgccgtgt
      241 ggcagacccc ctaaaaaggc tgtttaagct tggcaaacct ctggcagcag acgatgaaca
      301 tgatgatgac aggagaaggg cattgcatga agagtcaaca cgctggaacc gagtgggtat
      361 tctttcagag ctgtgcaagg cagtagaatc aaggtatgaa accgtaggaa cttccatcat
      421 agttatggcc atgactactc tagctagcag tgttaaatca ttcagctacc tgagaggggc
      481 ccctataact ctctacggct aacctgaatg gactacgaca tagtctagtc cgccaagtct
      541 agcatatggg cgcgtgaatt cgccaccatg gcgggacacc tggcttcgga tttcgccttc
      601 tcgccccctc caggtggtgg aggtgatggg cca
//
