LOCUS       sequence-96812-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT with PRAI insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Hsiau TH, Sukovich D, Elms P, Prince RN, Strittmatter T, Ruan P, 
            Curry B, Anderson P, Sampson J, Anderson JC
  TITLE     A method for multiplex gene synthesis employing error correction 
            based on expression.
  JOURNAL   PLoS One. 2015 Mar 19;10(3):e0119927. doi: 
            10.1371/journal.pone.0119927. eCollection 2015.
  PUBMED    25790188
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-03-19"; volume: "10(3):e0119927. doi"; pages: " 
            10.1371/journal.pone.0119927. eCollection 2015"
COMMENT     Sequence Label: pSALECT-PRAI
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..90
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(105..110,111..128,129..135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
       61 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      121 ccggctcgta tgttgtgtgg aattgtgagc gggataacaa tttcacacag gatctcgaca
      181 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
      241 taaccgtcgc cgggatgctg gggccgtcat tgctaacgcc gcgacgtgcg actgcgcata
      301 tggaattcat ggttagagtt aaaatctgcg gtattactaa ccttgaggac gcactttttt
      361 ccgtagaatc tggggctgat gctgtcggat tcgtttttta tcctaaaagt aagagatata
      421 tcagtcccga agatgccaga aggatctccg tggaactgcc gccgtttgtc ttcagggtgg
      481 gtgtcttcgt caatgaggag cctgagaaaa tcctggatgt ggcctcgtat gtgcagttga
      541 atgctgtcca gctacatgga gaagaaccta tagagctttg tcgtaaaatt gccgaaagga
      601 tcttggtgat taaagctgtt ggtgtctcta acgaaagaga catggagagg gcgctaaact
      661 accgtgaatt tcccatttta cttgacacta aaactcctga atatggtgga tctggtaaaa
      721 catttgattg gtcactgatc ctgccttaca gagaccgttt cagatatcta gtgttaagcg
      781 gcggcct
//
