LOCUS       sequence-96818-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Pomper MG, Hammond H, Yu X, Ye Z, Foss CA, Lin DD, Fox JJ, Cheng L
  TITLE     Serial imaging of human embryonic stem-cell engraftment and teratoma
            formation in live mouse models.
  JOURNAL   Cell Res. 2009 Mar;19(3):370-9.
  PUBMED    19114988
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res.";
            date: "2009-03"; volume: "19(3)"; pages: "370-9"
COMMENT     Sequence Label: cEF.tk-GFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           89..128
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
ORIGIN
        1 gtgaggaatt agcttggtac taatacgact cactataggg agacccaagc tggctaggta
       61 agcttgatgc taccggactc agatcccgga tcttggtggc gtgaaactcc cgcacctctt
      121 cggcaagcgc cttgtagaag cgcgtatggc ttcgtacccc tgccatcaac acgcgtctgc
      181 gttcgaccag gctgcgcgtt ctcgcggcca tagcaaccga cgtacggcgt tgcgccctcg
      241 ccggcagcaa gaagccacgg aagtccgcct ggagcagaaa atgcccacgc tactgcgggt
      301 ttatatagac ggtcctcacg ggatggggaa aaccaccacc acgcaactgc tggtggccct
      361 gggttcgcgc gacgatatcg tctacgtacc cgagccgatg acttactggc aggtgctggg
      421 ggcttccgag acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat
      481 atcggccggg gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta
      541 tgccgtgacc gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc
      601 cccgcccccg gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta
      661 cccggccgcg cgatacctta tgggcagcat gacccccca
//
