LOCUS       sequence-96822-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PDB 4O2Z.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     SGC_Toronto_Q4_2013_Q1_2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MAC04E-A09
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             172..192
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatataccat gcatcatcat catcatcaca gcagcggcag agaaaacttg
      181 tatttccagg gcaaccagga gctgagcgtg cccaagattg tgggggactt caaggtgtac
      241 aatgtgagcg gctccccatt cgaggttccg tccaagtaca cgctgctcaa gatcctcggc
      301 atgggagcct acggtatcgc gtgcagctgc ttggatggcg acactggcga gaaggtgtcc
      361 attaagaagt gtcgcgacgt gttccgcgat gtggaggatg gcaaacgagt gctgcgcgag
      421 atcgatatga tgcgcttctt ccaccacgaa aacctgctca atgtggtgaa cattttgcct
      481 ccgctgaagc gcgagtacca cagcttcgaa gacgtgtacg tcgtcacccc gctcatggac
      541 gtagacatga atgtcgtact gcgctctcgt caggtgctcg aggagtcgca catgcagtac
      601 tttgtctacc agattctgcg tggcctcaag tacctacaca gcgcgaacgt cgcccaccgc
      661 gacttgaagc cagccaacct cgtcacaaac atctcttgtg agctcaagat tatagacttc
      721 gggctgagcc gcagcgtcga tgtgccgtac tcggagctca cggattacgt catcacgcgc
      781 tggtaccggc caccggagct gcttctggag
//
