LOCUS       sequence-96826-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from 
            bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pGEX6P1-HA-MEK5 CBM-E
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..67
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agttctgttc caggggcccc tgggatccgc cgccaccatg tacccatacg atgttccaga
       61 ttacgctctg tggctagccc ttggcccctt tcctgccatg gagaaccagg tgctggtaat
      121 tcgcatcaag atcccaaata gtggcgcggt ggactggaca gtgcactccg ggccgcagtt
      181 actcttcagg gatgtgctgg atgtgatagg ccaggttctg cctgaagaaa caactacagc
      241 atttgaatat gaagatgaag atggtgatcg aattacagtg agaagtgatg aggaaatgaa
      301 ggcaatgctg tcatattatt attccacagt aatggaacag caagtaaatg gacagttaat
      361 agagcctctg cagatatttc caagagcctg caagcctcct ggggaacgga acatacatgg
      421 cctgaaggtg aatactcggg ccggaccctc tcaacacagc agcccagcag tctcagattc
      481 acttccaagc aatagcttaa agaagtcttc tgctgaactg aaaaaaatac tagccaatgg
      541 ccagatgaat gaacaagaca tacgatatcg ggacactctt ggtcatggca acggaggcac
      601 agtctacaaa gcatatcatg tcccgagtgg gaaaatatta gctgtaaagg tcatactact
      661 agatattaca ctggaacttc agaagcaaat tatgtctgaa ttggaaattc tttataagtg
      721 cgattcatca tatatcattg gattttatgg agcatttttt gtagaaaaca ggatttcaat
      781 atgtacagaa ttcatggatg ggggatcttt ggatgtatat aggaaaatgc cagaacatgt
      841 cctt
//
