LOCUS       sequence-96829-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LAMP1-phLuorin-N-8
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             149..856
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     562..581
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 caaaggatcc aacaacatgt tgatccccat tgctgtgggc ggtgccctgg cagggctggt
       61 cctcatcgtc ctcattgcct acctcattgg caggaagagg agtcacgccg gctatcagac
      121 catctcaggg tctggaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc
      181 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
      241 gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct
      301 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
      361 gtacggctcc aaggtctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc
      421 cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca
      481 cgttaaccag gactcctccc tgcaggacgg cgtgttcatc tacaaggtga agctgcgcgg
      541 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      601 cgaggagcgg atgtaccccg aggacggcgc cctgaagagc gagatcaaga agaggctgaa
      661 gctgaaggac ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc
      721 cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga
      781 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
      841 ggacgagctg tacaagt
//
