LOCUS       sequence-96832-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-LAMP1-phLuorin-N-8
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..141
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     primer_bind     complement(169..197)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagcgccac catggacagc
       61 aaaggttcgt cgcagaaagg gtcccgcctg ctcctgctgc tggtggtgtc aaatctactc
      121 ttgtgccagg gtgtggtctc cagtaaagga gaagaacttt tcactggagt tgtcccaatt
      181 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      241 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      301 gttccatggc caacacttgt cactacttta acttatggtg ttcaatgctt ttcaagatac
      361 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      421 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      481 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      541 aacattcttg gacacaaatt ggaatacaac tataacgatc accaggtgta catcatggca
      601 gacaaacaaa agaatggaat caaagctaac ttcaaaatta gacacaacat tgaagatgga
      661 ggcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg gcccgtcctt
      721 ttaccagaca accattacct gtttacaact tctactcttt cgaaagatcc caacgaaaag
      781 agagaccaca tgg
//
