LOCUS       sequence-96838-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against human CTR1 from puromycin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pSUPER-retro-puro-tetO-CTR1-shRNA
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..50
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     35..51
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(185..204)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(385..408)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tccttctcta ggcgccggaa ttagatcgat ctctcgaggt cgacggtatc gataagctta
       61 aaaaaaagcc cagctttctc tttggtctct tgaaccaaag agaaagctgg gctttgggga
      121 tctctatcac tgatagggaa cttataagat tcccaaatcc aaagacattt cacgtttatg
      181 gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc ccctgtccct
      241 cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg
      301 cccgcgattc cttggagcgg gttgatgacg tcagcgttcg aattctaccg ggtaggggag
      361 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      421 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      481 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      541 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      601 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      661 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      721 ggcgggctca ggggcgg
//
