LOCUS       sequence-96841-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter gene with mouse c-fos promoter. Induced by serum and many 
            growth factors in quiescent cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator 
            for serum response factor (SRF), is required for serum induction of 
            SRF target genes.
  JOURNAL   Mol Cell Biol. 2003 Sep . 23(18):6597-608.
  PUBMED    12944485
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Sep . 23(18):6597-608."
COMMENT     Sequence Label: pFOS WT-GL3
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..118
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    611..702
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(643..662)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    716..764
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(1032..1053)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt taccaacagt
       61 accggaatgc caagctgatc tcgagcccgg gctagagaga cactggtggg agctgcagag
      121 cagagctggg tgggagcgcg gtcactgctc gttcgcggaa ccgccggctc tatccagtct
      181 tctcagttgc tcgctgcagt cgcggttgga gtagtaggcg cctcagctgg cgcctttata
      241 gaagcgctgt gaatggatgg acttcctacg tcactgggcg gaactggaag gggggggaag
      301 cgcgccccaa ggccctctgc gggcgctctg tcgtcaactc tacgccccag gctttcaacc
      361 cccgtcttgg catacatctt tcacctatga gtgttcacat ttgggatctt agggggtctc
      421 ctagaccttc cgcgtgtagg atttcggaga tggtcccccc cccagaacaa cagggaccgg
      481 ccgtggaaac ctgctgacgc agatgtccta atatggacat cctgtgtaaa ggagggaggg
      541 attgacggga acagctcgcc ggcggtcgac ctgcagccca agctcgcgta agagctcggt
      601 acctatcgat agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct
      661 agtttgtttt gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac
      721 aaaaaaccaa cacacagatg taatgaaaat aaagatattt tattgcggcc gctccaagta
      781 cctcccgtac cttaatatta cttacttatc atggtagctt gggctggcgt aatagcgaag
      841 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg
      901 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
      961 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1021 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1081 aagggcgaaa a
//
