LOCUS       sequence-96842-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter gene with mouse c-fos promoter. Induced by serum and many 
            growth factors in quiescent cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Cen B, Selvaraj A, Burgess RC, Hitzler JK, Ma Z, Morris SW, Prywes R
  TITLE     Megakaryoblastic leukemia 1, a potent transcriptional coactivator 
            for serum response factor (SRF), is required for serum induction of 
            SRF target genes.
  JOURNAL   Mol Cell Biol. 2003 Sep . 23(18):6597-608.
  PUBMED    12944485
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2003 Sep . 23(18):6597-608."
COMMENT     Sequence Label: pFOS WT-GL3
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(508..519)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      578..587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(650..668)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccagaacatt tctctatcga taggtaccga gctcttacgc gagcttgggc tgcaggtcga
       61 ccgccggcga gctgttcccg tcaatccctc cctcctttac acaggatgtc catattagga
      121 catctgcgtc agcaggtttc cacggccggt ccctgttgtt ctgggggggg gaccatctcc
      181 gaaatcctac acgcggaagg tctaggagac cccctaagat cccaaatgtg aacactcata
      241 ggtgaaagat gtatgccaag acgggggttg aaagcctggg gcgtagagtt gacgacagag
      301 cgcccgcaga gggccttggg gcgcgcttcc ccccccttcc agttccgccc agtgacgtag
      361 gaagtccatc cattcacagc gcttctataa aggcgccagc tgaggcgcct actactccaa
      421 ccgcgactgc agcgagcaac tgagaagact ggatagagcc ggcggttccg cgaacgagca
      481 gtgaccgcgc tcccacccag ctctgctctg cagctcccac cagtgtctct ctagcccggg
      541 ctcgagatca gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa
      601 cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact
      661 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
      721 tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc
      781 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
      841 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
      901 cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt
      961 gttcgtttcc aaaaa
//
