LOCUS       sequence-96848-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the POT family transporter from Streptococcus thermophilus
            in E. coli as a C-terminally tagged GFP his tagged protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S
  TITLE     Alternating access mechanism in the POT family of oligopeptide 
            transporters.
  JOURNAL   EMBO J. 2012 Aug 15;31(16):3411-21. doi: 10.1038/emboj.2012.157. 
            Epub 2012 Jun 1.
  PUBMED    22659829
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2012.157"; journalName: "EMBO J"; date: "2012-08-15- 
            15"; volume: "31"; issue: "16"; pages: "3411-21"
COMMENT     Sequence Label: pWaldo-GFPd_PepTSt
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgga agacaaagga
       61 aagacatttt tcgggcaacc tttaggactg tcaacactat tcatgactga aatgtgggag
      121 cgtttctcat actatggtat gcgcgccatc ttgctttact atatgtggtt cctcatcagt
      181 actggtgatc tacacattac acgagcaaca gccgcatcaa tcatggcaat ttacgcttca
      241 atggtttacc tttctggtac gatcggtggt ttcgtagccg atcgtatcat cggagcacgt
      301 ccagctgtat tctggggtgg tgttctcatc atgcttggac atatcgtgct cgctttgcct
      361 tttggcgcaa gcgcattgtt tgggtcaatc atcttaatta ttataggaac tggtttcttg
      421 aagccaaacg tttcaacatt ggttgggaca ttgtatgacg aacatgatcg tcgtcgtgac
      481 gctggtttct caatctttgt gtttggtatt aacttgggtg cgttcattgc accacttatt
      541 gttggtgctg cacaagaagc tgcaggttac cacgtggcct tctcattggc agcaatcggt
      601 atgtttattg gtttgctggt ttattacttc ggtggtaaga aaacattgga tccgcattac
      661 ttgcgcccaa ctgatccact agcaccagaa gaagttaagc cattactggt taaggtaagc
      721 ttagctgttg ctggctttat tgcaatcatc gttgtgatga accttgtcgg ttggaattca
      781 ttacctgcct acatcaatct cttgacgatt gttgcgatcg ccattccggt tttctatttt
      841 gcttggatga tttcttcagt gaaggtgacg tcaactgaac atttgcgtgt tgtgtcttac
      901 atcccacttt tcatcgctgc tgttttgttc tgggcgattg aagaacaagg ctcagttgtg
      961 ttggcaacat ttgctgctga acgtgttgat tcatcttggt tccctgtctc atggttccaa
     1021 tcattgaacc c
//
