LOCUS       sequence-96862-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral shRNA vector for knockdown of human PIK3CD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Bajikar SS, Fuchs C, Roller A, Theis FJ, Janes KA
  TITLE     Parameterizing cell-to-cell regulatory heterogeneities via 
            stochastic transcriptional profiles.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):E626-35. doi: 
            10.1073/pnas.1311647111. Epub 2014 Jan 21.
  PUBMED    24449900
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1311647111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-4- 4"; volume: "111"; issue: "5"; pages: "E626-35"
COMMENT     Sequence Label: pLKO.1 shPIK3CD.v1 puro
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    138..255
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        304..814
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     733..751
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(836..855)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggcgccaag atgtgccaat
       61 tctgctgcag cagaattggc acatcttggc gtttttgaat tctcgacctc gagacaaatg
      121 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      181 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      241 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
      301 gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
      361 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
      421 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
      481 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
      541 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
      601 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
      661 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
      721 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
      781 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggagc ttaccatgac
      841 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
      901 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgc
//
