LOCUS       sequence-96868-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DCatch encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyCatcher, for generation of chimeric 
            SpyAvidin tetramers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggctg aagctggtat caccggcacc
       61 tggtacgccc agctgggaga caccttcatc gttaccgctg gtgctgacgg tgctctgacc
      121 ggtacctacg aagccgctgt tggtgatgac gatggtgacg atgacggtga tgacgatgga
      181 gctgaatcta gatacgttct gaccggtcgt tacgactccg ctccggctac cgacggttcc
      241 ggaaccgctc tgggttggac cgttgcttgg aaaaacaact accgtaacgc tcactccgct
      301 accacctggt ctggccagta cgttggtggt gctgaagctc gtatcaacac ccagtggttg
      361 ttgacctccg gcaccaccga agctaacgcg tggaaatcca ccctggttgg tcacgacacc
      421 ttcaccaaag ttaaaccgtc cgctgcttca ggatcaggat caggagatag tgctacccat
      481 attaaattct caaaacgtga tgaggacggc aaagagttag ctggtgcaac tatggagttg
      541 cgtgattcat ctggtaaaac tattagtaca tggatttcag atggacaagt gaaagatttc
      601 tacctgtatc caggg
//
