LOCUS       sequence-96888-e         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-Tre for expression of traptavidin (high stability mutant of 
            streptavidin) bearing a glutamic acid tag for ion-exchange 
            chromatography.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Traptavidin-E6
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttttatta
       61 ctcttcctct tcctcttcgg aagcagcgga cggtttaact ttggtgaagg tgtcgtgacc
      121 aaccagggtg gatttccacg cgttagcttc ggtggtgccg gaggtcaaca accactgggt
      181 gttgatacga gcttcagcac caccaacgta ctggccagac caggtggtag cggagtgagc
      241 gttacggtag ttgtttttcc aagcaacggt ccaacccaga gcggttccgg aaccgtcggt
      301 agccggagcg gagtcgtaac gaccggtcag aacgtaatcg ccttcagcgt taccaacagc
      361 ggattcgtag gtaccggtca gagcaccgtc agcaccagcg gtaacgatga aggtggatcc
      421 cagctggttg taccaggtgc cggtgatacc agcttcagcc atatgtatat ctccttc
//
