LOCUS       sequence-96898-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag V5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_smFP V5
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..99
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     497..517
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(577..596)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          579..711
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagagccgca gagccggcag
       61 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
      121 cccgcgcaga atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc
      181 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
      241 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
      301 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
      361 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
      421 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
      481 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
      541 ttcacaaatt ttgtaatcca gaggttgatt gtcgacacct gtggagagaa aggcaaagtg
      601 gatgtcagta agaccaatag gtgcctatca gaaacgcaag agtcttctct gtctcgacaa
      661 gcccagtttc tattggtctc cttaaacctg tcttgtaacc ttgatactta cctgcggcct
      721 cgacctttag ctattcatcg gtacctcgag ca
//
