LOCUS       sequence-96899-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag OLLAS ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP OLLAS
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      150..159
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             162..203
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             213..254
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             264..305
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagcct cctgggcaac gtgctggtta ttgtgctgtc
       61 tcatcatttt ggcaaagaat tcggtaccgc gggcccggcg cgccagctgg atatctccgg
      121 acccgggcta gcgcaattgc ggccgcgccg ccaccatggg gtccggattt gccaatgaac
      181 ttggacctag acttatgggt aaacagcagc aatcaggctt tgccaacgag ttgggccctc
      241 ggttgatggg aaagcagcag cagtccgggt tcgccaacga actgggcccg aggctcatgg
      301 ggaagggcca gggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
      361 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
      421 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
      481 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
      541 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
      601 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
      661 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
      721 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctga
//
