LOCUS       sequence-96901-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag OLLAS ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP OLLAS
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..100
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     498..518
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(578..597)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          580..712
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagatgccgc agagccggca
       61 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
      121 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc
      181 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
      241 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
      301 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
      361 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
      421 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
      481 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
      541 tttcacaaat tttgtaatcc agaggttgat tgtcgacacc tgtggagaga aaggcaaagt
      601 ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca
      661 agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt acctgcggcc
      721 tcgaccttta gctattcatc ggtacctcga gcaccggtct atttgcccat cagttct
//
