LOCUS       sequence-96905-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             152..175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             179..202
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             758..781
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
       61 atcattttgg caaagaattc ggtaccgcgg gcccggcgcg ccagctggat atctccggac
      121 ccgggctagc gcaattgcgg ccgccaccat ggactacaag gacgacgacg ataaaggaga
      181 ttacaaagat gacgatgata agggcgatta taaggacgac gatgacaagg gcggcgtgtc
      241 gaaaggcgag gagctgttca caggcgtggt ccccatcctg gttgagctgg acggtgacgt
      301 taacggccac aaatttagcg tacgtggtga gggcgagggt gacgctacca acggaaagct
      361 gaccctgaaa ttcatctgca caactggcaa gctgccggtg ccctggccca cgctggtaac
      421 cactctgggc ggaggcgttc aatgtttcag tcgttacccg gatcacatga agcagcatga
      481 cttcttcaag tcggccatgc ccgaaggcta cgtgcaggag cgcaccatca gttttaagga
      541 tgacggcacc tacaagactc gcgccgaggt caagttcgaa ggagacaccc tggttaatcg
      601 tatcgagctc aaaggcatcg atttcaagga ggacggaaat attctgggcc acaagctgga
      661 gtacaatttt aacagccaca atgtttacat tactgccgac aagcagaaga acggcatcaa
      721 ggctaatttc aagattcgtc acaatgtgga gggaggcgac tataaggatg acgatgacaa
      781 acaacaagac
//
