LOCUS       sequence-96909-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             158..181
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             185..208
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             212..235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             740..763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
       61 catcattttg gcaaagaatt cggtaccgcg ggcccggcgc gccagctgga tatctccgga
      121 cccgggctag cgcaattgcg gccgcgccgc caccatggac tacaaggacg acgacgacaa
      181 aggtgactac aaagatgatg acgataaagg cgactataag gacgatgacg acaagggcgg
      241 aaactcactg atcaaggaaa acatgcggat gaaggtggtg atggagggct ccgtgaatgg
      301 tcaccagttc aagtgcaccg gagagggaga gggaaacccg tacatgggaa ctcagaccat
      361 gcgcattaag gtcatcgaag gaggtccgct gccgttcgct ttcgatatcc tggccacttc
      421 gttcggagga gggtcgcgca cgttcatcaa gtacccgaag ggaatcccgg acttctttaa
      481 gcagtcattc ccggaaggat tcacttggga acgggtgacc cggtatgaag atggaggtgt
      541 ggtgactgtc atgcaagata cttcgctgga ggatgggtgc ctcgtgtacc acgtccaagt
      601 ccgcggagtg aatttcccgt ccaacggacc agtgatgcag aaaaagacga agggttggga
      661 acctaatact gaaatgatgt accccgcaga cggagggctg aggggctaca cccacatggc
      721 gctgaaggtc gacggaggag attacaagga tgacgacgat aagcaacaag attacaaaga
      781 cgatgatgac
//
