LOCUS       sequence-96910-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes multiple copies of an epitope tag FLAG ( not detected by 
            anti GFP ab).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Viswanathan S, Williams ME, Bloss EB, Stasevich TJ, Speer CM, Nern 
            A, Pfeiffer BD, Hooks BM, Li WP, English BP, Tian T, Henry GL, 
            Macklin JJ, Patel R, Gerfen CR, Zhuang X, Wang Y, Rubin GM, Looger 
            LL
  TITLE     High-performance probes for light and electron microscopy.
  JOURNAL   Nat Methods. 2015 Jun;12(6):568-76. doi: 10.1038/nmeth.3365. Epub 
            2015 Apr 27.
  PUBMED    25915120
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3365"; journalName: "Nat Methods"; date: "2015-06"; 
            volume: "12"; issue: "6"; pages: "568-76"
COMMENT     Sequence Label: pCAG_mRuby2_smFP FLAG
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..102
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     500..520
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(580..599)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gggagatccg actcgtctga gggcgaaggc gaagacgcgg caccaggccg cagagccggc
       61 agcaggccgc gggaaggaag gtccgcctgg attgagggcc gaagggacgt agcagaagga
      121 cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc catggaaagg acgtcagctt
      181 ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
      241 aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga
      301 aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt
      361 gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca
      421 ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg
      481 cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa
      541 tctttcacaa attttgtaat ccagaggttg attgtcgaca cctgtggaga gaaaggcaaa
      601 gtggatgtca gtaagaccca ataggtgccc tatcagaaac gcaagagtct tctctgtctc
      661 gacaagccca gtttctattg gtctccttta aactgtcttg taaacctttg atactttacc
      721 ctgcggcctc g
//
