LOCUS       sequence-96924-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pPAmCherry1-N1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..815
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(250..268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      121 aggaggataa catggccatc attaaggagt tcatgcgctt caaggtgcac atggaggggt
      181 ccgtgaacgg ccacgtgttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
      241 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcacc tgggacatcc
      301 tgagccctca gttcatgtac ggctccaatg cctacgtgaa gcaccccgcc gacatccccg
      361 actactttaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aaattcgagg
      421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
      481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca
      541 tgggctggga ggccctctcc gagcggatgt accccgagga cggcgccctg aagggcgagg
      601 tcaagcccag agtgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
      661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaaccgc aagctggaca
      721 tcaccagcca caacgaggac tacaccatcg tggagcagta cgagagagcc gagggccgcc
      781 actccaccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
      841 agccatacca catttgtaga ggttttac
//
