LOCUS       sequence-96927-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: pBAD-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     111..128
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(174..875)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(885..902)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
       61 cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct
      121 gtatcaggct gaaaatcttc tctcatccac caaaacagcc aagcttcgaa ttcttaatta
      181 agcttgtgcc ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc
      241 tcgacgtagg tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag
      301 acgccaggca tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttggcgttt
      361 gcgatcagat ggctcccgcc cacgagcttc agggccatgt cgtttctgcc ttccaggccg
      421 ccgtcagcgg ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc
      481 acagggccgt tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg
      541 tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact
      601 ctctcccatg tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg
      661 tggttgatga aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag
      721 gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc gtagggcttg
      781 ccttcgccct cggatgtgca cttgaagtga tggttgtcca cggtgccctc catgtacagc
      841 ttcatgtgca tgttctcctt aatcagctcg ctcatagatc tgccatggtg atggtgatgg
      901 tggctcccca tggttaattc ctcctgttag
//
