LOCUS       sequence-96938-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA for cleavage at rde-1(D718) locus in C elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ
  TITLE     Efficient Marker-Free Recovery of Custom Genetic Modifications with 
            CRISPR/Cas9 in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Aug 26. pii: genetics.114.169730.
  PUBMED    25161212
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Aug 26. pii: genetics.114.169730."
COMMENT     Sequence Label: pJA45
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(31..133)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(134..165)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     213..229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     213..229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(372..447)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(468..817)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
ORIGIN
        1 gcaagacgtt tcccgttgaa tatggctcat tttagcttcc ttagctcctg aaaatctcga
       61 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      121 tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
      181 caggtatgat ttaaatggtc agtattgagc ctcaggaaac agctatgaca tcaagctgac
      241 tagataatct agctgatcgt ggaccgatca tacgtataat gccgtaagat cacgggtcgc
      301 agcacagctc gcggtccagt agtgatcgac actgctcgat ccgctcgcac cgctagcaaa
      361 tttcacaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
      421 ttaacttgct atttctagct ctaaaacaca tacatagtta atggcacaag acatctcgca
      481 ataggaggtg tttatatagg ggcaacacaa gaggcggcag acatctcgtg agtttgacag
      541 agtagtataa agagtgtgtg tgagagggtg taggcatttc ataacatatt ttttgtggaa
      601 taattttgga aaaaaggtgt ttttttaata atagctttta taagagtttc tcaaaaattg
      661 catgcttttt ttgaaaaggt tgacaaatta tagtagtact aaagttgcta ttttcaagtc
      721 tcagcagaaa attcacaact aaaaataaaa tttgcaaaac agagtgtgcg cctttaaggt
      781 gtacagtttt ttattccttt attgctagtt ttttttgaag cttggatcga cgagagcagc
      841 gcgactggat ctgtcgcccg tctcaaacgc aa
//
