LOCUS       sequence-96957-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty vector for gRNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ
  TITLE     Efficient Marker-Free Recovery of Custom Genetic Modifications with 
            CRISPR/Cas9 in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Aug 26. pii: genetics.114.169730.
  PUBMED    25161212
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Aug 26. pii: genetics.114.169730."
COMMENT     Sequence Label: pRB1017
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..51
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     35..51
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        226..575
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     misc_RNA        619..694
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(837..853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cctttgattt tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt
       61 cagcttgatt ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag
      121 ttacgctgga gtctgaggct cgtcctgaat gatatgcgac cgccggaggg ttgcgtttga
      181 gacgggcgac agatccagtc gcgctgctct cgtcgatcca agcttcaaaa aaaactagca
      241 ataaaggaat aaaaaactgt acaccttaaa ggcgcacact ctgttttgca aattttattt
      301 ttagttgtga attttctgct gagacttgaa aatagcaact ttagtactac tataatttgt
      361 caaccttttc aaaaaaagca tgcaattttt gagaaactct tataaaagct attattaaaa
      421 aaacaccttt tttccaaaat tattccacaa aaaatatgtt atgaaatgcc tacaccctct
      481 cacacacact ctttatacta ctctgtcaaa ctcacgagat gtctgccgcc tcttgtgttg
      541 cccctatata aacacctcct attgcgagat gtcttggaga ccggtaacca tggctcgaga
      601 aaccggtact cggtctctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      661 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttg tgaaatttgc tagcggtgcg
      721 agcggatcga gcagtgtcga tcactactgg accgcgagct gtgctgcgac ccgtgatctt
      781 acggcattat acgtatgatc ggtccacgat cagctagatt atctagtcag cttgatgtca
      841 tagctgtttc ctgaggctca atactgacca ttt
//
