LOCUS       sequence-96971-e        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for Drosophila melanogaster Or65a expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Vosshall LB, Amrein H, Morozov PS, Rzhetsky A, Axel R
  TITLE     A spatial map of olfactory receptor expression in the Drosophila 
            antenna.
  JOURNAL   Cell. 1999 Mar 5;96(5):725-36.
  PUBMED    10089887
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1999-03-5"; volume: "96(5)"; pages: "725-36"
COMMENT     Sequence Label: pGEM-T Dmel Or65a
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(745..763)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(746..763)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(781..797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(781..797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(794..816)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    805..821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(829..835,836..853,854..859))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    874..895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1012..1029)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
       61 gccgcgggat tttggaccag aatgacaggt gacccagagt cattaacata ataatcatat
      121 actcggctgc aacttgggga ttcctcttgg ctgccagcac ttcgaaaagt gacaatgaga
      181 cgagcagaaa gttaatcaac atttgcatga taaaagctcc attgaatata tggttgcagc
      241 gatctgtaag tagtatgatt tgctgatgga acttggtcat tcgacaaagc tctctgtaca
      301 gcatatcttc atcgcccagg caaagttcct gaagatttct tagttcgatg cataaaaccc
      361 ttagagcaga agttagctca aaaaataaag acacacccat attctcaaca acaccaagcc
      421 aaatcaggaa gtacaacatc gtggtgcttt gggatacaaa gatgatgata taggcaatcg
      481 gatgtttcga tggatcgtga agttgaaagg gccaagagac gtgaaagggt aatgtctgcg
      541 attcaatcca aaatggtgtg gatattttta tcagcataaa aagtatgaga aagctgaacc
      601 aggtaatgat caaggccata aacaatagga aatgcaaacg cttggtttct tgcacttcct
      661 ttgttgcagg aatcactagt gcggccgcct gcaggtcgac catatgggag agctcccaac
      721 gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
      781 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      841 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      901 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      961 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1021 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1081 aatacggt
//
