LOCUS       sequence-97-expo        7406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_25
FEATURES             Location/Qualifiers
     source          1..7406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             527..547
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(637..659)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4846..4868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4968..4987
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5065..5083)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5151..5255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5256..5324,5325..6116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5474..5493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6287..6875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6776..6795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7029..7046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7163..7184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7199..7204,7205..7222,7223..7229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7237..7253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7242..7264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7261..7277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7261..7277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc cttggtcgac tctagaggat ccccggggat tggccaaagg
       61 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
      121 gcttggctag caaaaatgca taaggttttg ctggcactgt tctttatctt tctggcacca
      181 gcatccgcac tggcagtctc cgaaccggcc tgcaggatcg attttttgca aattacgagc
      241 gttgtagggg gcggagcgat aggtcctata ggttttggta tatcatcatt cattcattca
      301 ttggtacatt catttaccca ccttcctctt tctgagcttc tctggagttc tgtgcttcct
      361 ttttccctta tctttatact gtaattttta actttcaggc attgattgga tccccgggat
      421 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      481 gaggaccctt gcttggaggg taccgagctc agaaaaaatg actgctccaa agaagaagcg
      541 taaggtaccg gtgggtgaag accagaaaca gcacctcgaa ctgagccgcg atattgccca
      601 gcgtttcaac gcgctgtatg gcgagatcga tcccgtcgtt ttacaacgtc gtgactggga
      661 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      721 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      781 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga
      841 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc
      901 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga
      961 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg
     1021 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg
     1081 ctgggtcggt tacggccagg taagtttaat taagttgata ctaactaaca agatctgatt
     1141 aattttcagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     1201 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
     1261 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     1321 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     1381 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     1441 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     1501 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     1561 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     1621 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     1681 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     1741 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatgtaag tttaaactat
     1801 tcgttactaa ctaactttaa acatttaaat tttcagatcc tgctgatgaa gcagaacaac
     1861 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac
     1921 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
     1981 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg
     2041 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tggtaagttt
     2101 aaacagttga atactaacta acggagatct ttgaaatttt cagaatcagg ccacggcgct
     2161 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     2221 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     2281 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     2341 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     2401 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     2461 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     2521 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     2581 gtctttgccg accgcacgcc gcatccagcg taagtttaaa caataaccta ctaactaacg
     2641 tagataattt aaattttcag gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     2701 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     2761 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     2821 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     2881 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     2941 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     3001 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     3061 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     3121 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     3181 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcggtaag
     3241 tttaaacaaa gttgtactaa ctaacgaaga tcttgataat tttcagaacg ctggaaggcg
     3301 gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat
     3361 gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc
     3421 cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg
     3481 gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca
     3541 gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc
     3601 gcctgttttg accgctggga tctgccattg tcagacatgt agtaagttta aacttgatag
     3661 tactaactaa catgtttcat ttaaattttc agtaccccgt acgtcttccc gagcgaaaac
     3721 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
     3781 cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg
     3841 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc
     3901 gacgactcct ggagcccgtc agtatcggcg gaattccaac tgagcgccgg tcgctaccat
     3961 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     4021 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     4081 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     4141 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     4201 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     4261 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     4321 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     4381 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     4441 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     4501 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     4561 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     4621 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     4681 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     4741 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     4801 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     4861 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     4921 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     4981 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5041 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     5101 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6661 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6901 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7081 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7141 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7201 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7261 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     7321 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     7381 atacgctaac aacttggaaa tgaaat
//
