LOCUS       sequence-97007-e         488 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and 
            Lin28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S CK2M EN2L
FEATURES             Location/Qualifiers
     source          1..488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(84..113)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(357..383)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 acgcgtgggg gcggccaaac ttacgcacaa gagttccgta gctgttcaag tttgtgtttc
       61 aactgttctc gtcgtttccg caacaagtcc tcttcagaaa tgagcttttg ctcctctgct
      121 tggacggaca ggatgtatgc tgtggctttt ttaaggataa ctaccttggg ggccttttca
      181 ttgttttcca actccgggat ctggtcacgc agggcaaaaa agctccgttt tagctcgttc
      241 ctcctctggc gctccaagac gttgtgtgtt cgcctcttga cattctcctc ggtgtccgag
      301 gacctggggc tggtgcattt tcggttgttg ctgatctgtc tcaggactct gacactgtcc
      361 aacttgaccc tcttggcagc aggatagtcc ttccgagtgg agggaggcgc tgcgtagttg
      421 tgctgatgtg tggagacgtg gcacctcttg aggaccagtg ggctgtgagg aggtttgctg
      481 tggcctcc
//
