LOCUS       sequence-97016-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic excitation of ES cell-derived neurons (and other cells, 
            such as muscle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-CAG::ChR2-YFP
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(129..176)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    231..455
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     444..461
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(579..600)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttccttttca tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
       61 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      121 acctgacgga agttcctata ctttctagag aataggaact tcggaatagg aacttccccg
      181 ggttaattaa actagactcg agccccagct ggttctttcc gcctcagaag ccatagagcc
      241 caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc
      301 caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt
      361 ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg
      421 gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc gagctctagg
      481 gctagcgggc ccccatggga tccactagtg gatcccccgg gctgcaggaa ttcttacttg
      541 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      601 tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag gta
//
