LOCUS       sequence-97020-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for bacterial expression; His,GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  TITLE     Gateway Destination vectors for E. coli expression
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-565
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             130..152
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             169..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga
       61 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      121 agaaataatt ttgtttaact ttaagaagga gatatacata tgagatccca tcaccatcac
      181 catcacggcg cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
      241 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      301 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      361 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      421 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      481 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      541 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      601 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      661 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttccc
//
