LOCUS       sequence-97022-e         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector for bacterial expression; His,GST tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  TITLE     Gateway Destination vectors for E. coli expression
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-565
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(116..202)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(368..385)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(392..424)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    484..608
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc
       61 ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg
      121 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
      181 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc
      241 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
      301 accgcgctac tgccgccagg caaattctgt tttatcagcc taggaacgcc caacttaatt
      361 aacattagtg gtggtggtgg tggtgctcga gatcctctgg gtcttcagga gcgagttctg
      421 gctggcttgc acgtgtatac agctgtgcgg ccgcaagctt ctagctatct agctagcgat
      481 atcaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      541 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      601 tcactatgg
//
