LOCUS       sequence-97029-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion between the Lck domain and cytosolic GCaMP5G. 
            This construct targets GCaMP5G to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Akerboom J, Chen TW, Wardill TJ, Tian L, Marvin JS, Mutlu S, 
            Calderon NC, Esposti F, Borghuis BG, Sun XR, Gordus A, Orger MB, 
            Portugues R, Engert F, Macklin JJ, Filosa A, Aggarwal A, Kerr RA, 
            Takagi R, Kracun S, Shigetomi E, Khakh BS, Baier H, Lagnado L, Wang 
            SS, Bargmann CI, Kimmel BE, Jayaraman V, Svoboda K, Kim DS, 
            Schreiter ER, Looger LL
  TITLE     Optimization of a GCaMP calcium indicator for neural activity 
            imaging.
  JOURNAL   J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 
            10.1523/JNEUROSCI.2601-12.2012.
  PUBMED    23035093
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2601-12"; journalName: "J Neurosci"; date: 
            "2012-10-3- 3"; volume: "32"; issue: "40"; pages: "13819-40"
COMMENT     Sequence Label: pN1 Lck-GCaMP5G
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(125..568)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     684..703
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      121 ccgcttactt cgctgtcatc atttgtacaa actcttcgta gtttacctga ccatccccat
      181 cgatgtctgc ttccctgatc atttcatcaa cctcttcatc tgttaacttc tctccaaggt
      241 ttgtcatcac gtggcgaagc tctgctgcac tgatgtagcc attgccatcc ttatcaaaca
      301 cacggaacgc ttctctaatt tcttcttcac tgtctgtgta tttcattttt cttgccatca
      361 ttgtcaggaa ctcagggaag tcgattgtgc cgtcaccgtc ggcatctact tcattgatca
      421 tgtcctgcag ctctgcttct gtggggttct gccccagaga ccgcatcacc gtccccagct
      481 ccttggttgt tattgtccca tccccgtcct tgtcaaatag ggagaaagcc tctttaaatt
      541 ctgcgatctg ctcttcagtc agttggtccg gcaggttgta ctccagcttg tgccccagga
      601 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      661 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      721 gctcctggat gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      781 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacga
//
