LOCUS       sequence-97077-e         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVET-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(188..382)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(485..505)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaataccagt caatctttca caaattttgt aatccagagg ttgattatca tatgactagt
       61 cccgggaatt cgaagcttga gctcgactag ctagctagct actagctagt cgagatcctt
      121 aaactgatga tttgatttca aatgcagtct ctgaatcagg atgggtctct tggtgaattt
      181 ctctctccac cagccagggc tcttctccct tctccaaccg gaggatcaca tctggcttag
      241 taagctgata acccaaggaa accaggttct tatagttctc cagcatcaca tttctgtaca
      301 cgatctgctg agcagtgtcc agcagcttcc actcctccct ggtgaagtcc acaaatacat
      361 ccttgaaggt caccagtgtc cgggaccagg cagttagtga cttagcatcc atgccctcct
      421 tgttcttgat gatacttcct tgagtgacag cagagtgctg aggagacaaa gcaccaccgc
      481 cgtcgacctt tctcttcttt tttggcgacc cactttcaca tttaagttgt ttttctaatc
      541 cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata
      601 attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt
      661 ctttagcgac ttgatgctct tg
//
