LOCUS       sequence-97080-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP160 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC154-dual-dCas9VP160-sgExpression
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..392)
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(426..446)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcgatataca acatatccaa atcgaagtca tcgagcgcat cggagcccag catgtccagg
       61 tcaaagtcgt ccagggcatc actccccagc atatccaagt caaaatcatc caaggcatcg
      121 gaccccagca tgtccagatc gaaatcgtcc agggcgtcgc tgccgagcat gtccaggtcg
      181 aaatcatcaa gggcatcgct tcccaacata tcaaggtcga aatcatccag tgcgtctgac
      241 cctaacatat cgagatcgaa atcgtccaga gcatcggagc cgagcatgtc cagatcaaag
      301 tcatccaatg cgtcgcttcc caacatatcc aggtcaaagt catcgagggc atcagaaccc
      361 agcatgtcga gatcgaaatc gtccagcgcg tcggcgcgcc cggatccggc cggcccgctg
      421 gcctccacct ttctcttctt cttggggctg tcgcctccca gctgagacag gtcgatccgt
      481 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg
      541 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc
      601 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc
      661 cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc
      721 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccac
//
