LOCUS       sequence-97082-e         454 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP160 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC154-dual-dCas9VP160-sgExpression
FEATURES             Location/Qualifiers
     source          1..454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttccagatta cgcttcgccg aagaaaaagc gcaaggtcga agcgtccgac aagaagtaca
       61 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      121 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      181 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      241 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
      301 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
      361 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
      421 tggcctacca cgagaagtac cccaccatct acca
//
