LOCUS       sequence-97101-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Probe for Drosophila melanogaster Or46a expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Vosshall LB, Amrein H, Morozov PS, Rzhetsky A, Axel R
  TITLE     A spatial map of olfactory receptor expression in the Drosophila 
            antenna.
  JOURNAL   Cell. 1999 Mar 5;96(5):725-36.
  PUBMED    10089887
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1999-03-5"; volume: "96(5)"; pages: "725-36"
COMMENT     Sequence Label: pGEM-T Dmel Or46a
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(861..879)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(862..879)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(897..913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(897..913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(910..932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    921..937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(945..951,952..969,970..975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    990..1011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
       61 gccgcgggat ttagacgctc cacggttttc gacagttcaa cgatggtcat gtgatagcgg
      121 atattttcct ttagttgctg ttccacagtg ccaccagaag tagtgattaa ccgaccgatc
      181 ttgtccagtc ggacggccag aatgtccagc tggcacttga tgaagatgaa cagtgaggag
      241 catagtgagt cgaatcctat gttggtgatg caggatacgg acaaggccag acactgatag
      301 cagtagagga gccaataagc aggtgagccg gtattctcgc caagcggatt gtatgttttg
      361 agcggaaggt gggaccagtc caaggcgaac tggggtatga gaatcatgct taaggcggca
      421 aacgaaagcg ctccatagaa gtcccgaagc ttcctacttc tttcacaggc tgctgcaaag
      481 atcaactctt cgttggcgcc tctaggacgg aactcttcgt tatccaatct cctaaagagc
      541 tcctccatct gcacattgtt cgcctttatg gacagcagct tggtggtgtg ccctgcactg
      601 gtggccagga taaagatggc tttcacaacc tcggacatgt agctcaaatt agccatcaca
      661 tggagcacca agaggagatc aaagagcagc acccaaaaca caattacgtg gacatagttc
      721 acctggtgga tgattctcca ccggcgttct atctgaggcc acaacgagag gatgttcaag
      781 aatgccaatc actagtgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt
      841 tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
      901 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      961 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1021 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatt
//
