LOCUS       sequence-97106-e        4278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..4278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..25
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    67..100
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    122..155
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    177..210
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          350..415
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             545..565
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    837..971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(874..893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     928..947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1794..1827
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             1839..2552
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1869..1897)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2492..2512
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2553..2585
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    2605..2638
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             2643..3350
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             3351..3377
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3381..3407
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3411..3437
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    3463..3496
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4218..4259
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat
       61 agatctataa cttcgtataa agtatcctat acgaagttat gaggcagcgc ggctatcgtg
      121 gataacttcg tataatgtgt actatacgaa gttatctggc cacgacgggc gttcctataa
      181 cttcgtataa tgtatgctat acgaagttat gccctgaatg cggccgccag tgtgatggat
      241 atctgcagaa ttcgcccttc ggccgccaaa atggatcttt gtgaaggaac cttacttctg
      301 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
      361 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
      421 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
      481 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
      541 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
      601 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
      661 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc
      721 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      781 acctcccaca cctccctctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      961 atgtctggat ctgcgtcgcg ccacttcaac gctcgatggg agcgtcattg gtgggcgggg
     1021 taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa aaaattcact gcaacactga
     1081 aaagcatacg aaaacgatga agattgtacg aggaaccata aagtatttta tccacaaaga
     1141 cacgtatagc agaaaagcca agttaactcg gcgataagtt gtgtacacaa gaataaaatc
     1201 ggccagattc agtgttgtca gaaataagaa aaccccacta tgtttttctt tgccttttct
     1261 ttctcccagc gatcattcat ttcgtggtga aagaacgggg tcattgcacg gagtttcgac
     1321 tgcgggaaag cagagctgcc gttcacttcg tctataatta gcgctttcta ttttccccga
     1381 ttcgggccgc tgctgcgctt ttccgcctgc tgtttgtggc aagtgtagca gcaggctgtg
     1441 cacgcagtgt ggcatgcact tggctttcca ccgttggtat cgattctctg ggacgatgag
     1501 tcattccttt cggggccaca gcataatcgt tgccagctca ccgaaatggt gacttcattt
     1561 cttaactgcc gtcaagcatg cgattgtaca tacatacata tttatatatg tacatattta
     1621 tgtgactatg gtaggtcgat ataatagcaa tcaacgcaag caaatgtgtc agtcctgctt
     1681 acaggaacga ttctatttag taattttcgt tgtataaagt aattatgtat gtatgtaagc
     1741 cccataaatc tgaaacaatt aggcaaaacc atgcgaggat gaactgcaag acgataactt
     1801 cgtataaagt atcctatacg aagttatgcg gccgcaaaat gagtaaagga gaagaacttt
     1861 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1921 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1981 gcactactgg aaaactacct gttccatggc caacacttgt cactacttta acttatggtg
     2041 ttcaatgctt ttcaagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     2101 tgcccgaagg ttatgtccag gaaagaacta tatttttcaa agatgacggg aactacaaga
     2161 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     2221 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     2281 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     2341 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     2401 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     2461 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     2521 ggattacaca tggcatggat gaactataca aacagcccga gctggccccc gaggatcccg
     2581 aggattgacg taagctagct cgagataact tcgtataatg tgtactatac gaagttatca
     2641 aaatggtgag caagggcgag gagctcacaa tgggcgtaat caagcccgac atgaagatca
     2701 agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg
     2761 gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc
     2821 ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt
     2881 accccgacga catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc
     2941 gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg
     3001 aggactcctt catctacgag atacacctca agggcgagaa cttccccccc aacggccccg
     3061 tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg
     3121 gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc caccaccgcg
     3181 ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg
     3241 tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg
     3301 agagcgccgt ggcccgcaac tccaccgacg gcatggacga gctgtacaag tacccatacg
     3361 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga tatccatatg
     3421 acgttccaga ttacgctggc ggataacgta agctagggta ccataacttc gtataatgta
     3481 tgctatacga agttatcaaa atgataccgg tcggagtaag caaaggtgag gagagcgtga
     3541 tcaaacccga gatgaagatg cgctactata tggacggcag cgtcaacggc cacgagttta
     3601 ccatcgaggg cgagggcacg ggacgcccgt acgaaggaca ccaggagatg accctgcgcg
     3661 tgaccatggc cgagggtggc ccgatgccct tcgccttcga tctggtgagc cacgtgttct
     3721 gctacggtca ccgcgtcttc accaaatacc ccgaggaaat ccccgattac ttcaagcaag
     3781 ccttcccgga gggcctgtcg tgggagcgca gcctggagtt cgaggatggc ggaagcgcca
     3841 gcgtgagcgc gcacatcagc ctgcggggca acaccttcta ccataagagc aagttcaccg
     3901 gcgtgaattt cccagccgat ggccccatca tgcagaacca gagtgtggat tgggagccca
     3961 gcaccgagaa gatcaccgca agcgatggcg tgctgaaggg cgatgttacc atgtacctga
     4021 agctggaggg aggcggcaac cacaagtgcc agatgaaaac cacctacaag gccgccaagg
     4081 ggatcctgga gatgcccgga gatcactaca ttggccaccg cctggtccgc aagacagaag
     4141 gaaacatcac cgagcaggtg gaggatgccg tggcgcattc gggcatggat gagctgtaca
     4201 agggcgcgcc agctaatggc aagcccatcc ccaaccccct gctgggcctg gatagcacct
     4261 gacgtaagct agtctaga
//
