LOCUS       sequence-97116-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic excitation of ES cell-derived neurons (and other cells, 
            such as muscle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Bryson JB, Machado CB, Crossley M, Stevenson D, Bros-Facer V, 
            Burrone J, Greensmith L, Lieberam I
  TITLE     Optical control of muscle function by transplantation of stem 
            cell-derived motor neurons in mice.
  JOURNAL   Science. 2014 Apr 4;344(6179):94-7. doi: 10.1126/science.1248523.
  PUBMED    24700859
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2014-04-4- 4"; volume: "344"; issue: 
            "6179"; pages: "94-7"
COMMENT     Sequence Label: tol2-CAG::ChR2-YFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..47)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    72..119
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    124..171
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(183..202)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    248..303
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(302..321)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ccggggatcc tctaggggga ggctgactga aacacggaag gagacaatac cggaaggaac
       61 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
      121 taagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cgtcgaatca
      181 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
      241 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
      301 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
      361 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
      421 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
      481 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
      541 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
      601 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tctgcgaaga
      661 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
      721 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
      781 tccaggtcaa ggtgctgtgc attgtggtaa taga
//
