LOCUS       sequence-97117-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LG1
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggctcctcg ctggaggacc cactgcggag ctttgtgcga
      121 gttttggaga agcgggatgg tacagtgcta cgactacagc agtatagctc cggtggcgtg
      181 ggttgcgttg tgtgggacgc tgccattgtc ctttctaaat acctggaaac gcccgagttt
      241 tctggcgacg gggcccacgc gctgagccgg cggtcggtgc tggagctggg ttcgggcacc
      301 ggggccgtgg ggctcatggc tgctaccctc ggggctgatg ttgtagtcac cgatcttgag
      361 gaattgcaag acttgctgaa gatgaatatt aatatgaaca agcatcttgt cactggttct
      421 gttcaagcca aggtactgaa atggggggaa gaaatagaag gctttccttc tccacccgac
      481 ttcatactga tggccgactg catatactat gaagagtctt tggagccatt gctgaaaact
      541 ctaaaagata tcagcggatt tgaaacttgt attatatgtt gttatgaaca acgaacaatg
      601 gggaaaaatc cagaaattga gaaaaaatat tttgagctcc ttcagctaga ttttgacttt
      661 gaaaaaattc ctttggaaaa acatgatgaa gagtatcgaa gtgaagatat tcatattata
      721 tacatcag
//
