LOCUS       sequence-97120-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP
            tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
  TITLE     Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 
            and the Ubiquitin Ligase HERC2.
  JOURNAL   J Biol Chem. 2014 May 22. pii: jbc.M114.569392.
  PUBMED    24855649
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 May 22. pii: jbc.M114.569392."
COMMENT     Sequence Label: pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             189..254
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             264..287
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             294..314
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatactc cagcctccgg actctagcgc tagcacgcgt
      181 ccaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc gattacaagg
      241 atgacgatga caaggggtcg gcctggagcc acccgcagtt cgaaaaagga tccgagaacc
      301 tctacttcca aggctcgagt atgccctctg aatctttctg tttggctgcc caggctcgcc
      361 tcgactccaa atggttgaaa acagatatac agcttgcatt cacaagagat gggctctgtg
      421 gtctgtggaa tgaaatggtt aaagatggag aaattgtata cactggaaca gaatcaaccc
      481 agaacggaga gctccctcct agaaaagatg atagtgtcga accaagtgga acaaagaaag
      541 aagatctgaa tgacaaagag aaaaaagatg aagaagaaac tcctgcacct atatataggg
      601 ccaagtcaat tctggacagc tgggtatggg gcaagcaacc agatgtgaat gaactgaagg
      661 agtgtctttc tgtgctggtt aaagagcagc aggccctggc cgtccagtca gccaccacca
      721 ccctctcagc cctgcgactc aagcagaggc tggtgatctt ggagcgctat ttcattgcct
      781 tgaatagaac cgtttttcag gagaatgtca aagttaagtg gaaaagcagc ggtatttctc
      841 tgcctcctgt ggacaaaaa
//
