LOCUS       sequence-97125-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JUY
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..83
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             653..670
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(723..741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca
       61 gagaaaactt gtatttccag ggcatgccgg gggaggaaga ggagcgggcc ttcctggtgg
      121 cccgcgagga gctggcgagc gccctgagga gggattccgg gcaggcgttt tccctggagc
      181 agctccggcc gctactagcc agctctctgc cgctagccgc ccgctacctg cagctggacg
      241 ccgcacgcct tgtccgctgc aacgctcatg gggagccccg aaactacctc aacaccctgt
      301 ccacggctct gaacatcctg gagaaatacg gccgcaacct tctcagccct cagcggcctc
      361 ggtactggcg tggtgtcaag tttaataacc ctgtctttcg cagcacggtg gatgctgtgc
      421 aggggggccg agatgtgctg cgattatatg gctacacaga ggagcaacca gatgggttga
      481 gcttccccga agggcaggag gagccagatg agcaccaggt tgctacagtc acactggaag
      541 tactgctgct tcggacagag ctcagcctgc tattgcagaa tactcatcca agacagcagg
      601 cactggagca gctgttggaa gactgatgac gaagcttgcg gccgcactcg agcaccacca
      661 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      721 caccgctgag caataactag cataa
//
