LOCUS       sequence-97126-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of NPC1-CFP sterol sensing domain mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ko DC, Gordon MD, Jin JY, Scott MP
  TITLE     Dynamic movements of organelles containing Niemann-Pick C1 protein: 
            NPC1 involvement in late endocytic events.
  JOURNAL   Mol Biol Cell. 2001 Mar;12(3):601-14.
  PUBMED    11251074
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2001-03"; volume: "12(3)"; pages: "601-14"
COMMENT     Sequence Label: NPC1-CFP P692S
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(44..61)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccttgctcac catggtggcg atggatcccg ggcccgcggt accgtggtgg tggtggtggt
       61 gaaaattgag gagccgttct ctctctgtcc ctctgtagcg ctcgtaagtg gtgtgtcttt
      121 tagctttatt caccgacggc cctatgtaac tgagtaagac gggaagaaat attagtccat
      181 gagtggctcc gagtaagacc atggctaagt acatcctgaa gtaaaatatc tcaaaaattt
      241 gagatttggc aaaggctaac accacgatcc ctccaaattt cgtaagtgtg attccactga
      301 atacagaact acccatgtgg gccagtgcct cttccgcccg gctcactcgg cttcctttgg
      361 tactcatggt gaatgctctc gttatatggc tgcagaactc cacagaaatg ccgcagctca
      421 tcaccaagtt gaccaaggag accgcattca gactgatgcc ccacagccac atgacaccga
      481 acatgttgac caggatcatg gctatggtga tacacatgat gaccgcagac cacagctcac
      541 agcccagaac caccaaggtc accagaaata tggagcccag agacacactg aggttaaaga
      601 tggtgtcatc aataatggtc aggtactgtt catagaagac gtagaacaca ctgtaaggga
      661 atacgcggta gtcactcccc ttagaacgca tggtttccgt gatgttactg gctattagcc
      721 gagctttctt catggcatca gtatagtcag cggaggtctt aagtatggtg tggtaggtca
      781 tgaagtaagt ggccccaatg taagtgtcat ctcccacaat gttaacagct gaaccgtaag
      841 cagcatgtcc c
//
