LOCUS       sequence-97129-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4JWQ
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(617..637)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(650..667)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca
       61 agcttcgtca tcaatacttt agtttcatca tttcgcaaaa ctcataaaag tcgatctttc
      121 catcattgtt cttgtcaact tcttgaatca tttttttaac ttgattaaca tctttttgag
      181 ttatgcttcc tttcttgttg ccattataaa gtatatgtgc taattctgct gttgtaattt
      241 ccccatcatt gtctacatca aataccctaa aggcgcaata tatcagtttc tttgataaat
      301 gctttctgtc taatgctgcc gctaaaaatt cagtataatc tattctacca ctaccatcac
      361 tatctatttg atcaagtaat acatcaaaat tttgaggaag cttcaatcca ctattttcca
      421 aacctttttt caattgattt tttgttatat ttccttttcc atcttcatct aaatataaaa
      481 atactgtctt tagttgttgc aaatcgtagt cattactttg ttgtgctatt attgtcatag
      541 ctaatttttg aagttttaat aataaagcat aattcttaaa attttccaat acatgtatat
      601 ccattttgac atctaagccc tggaaataca agttttctct gccgctgctg tgatgatgat
      661 gatgatgcat ggtatatctc cttc
//
