LOCUS       sequence-97133-e         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses channel rhodopsin 2 (H134R) 
            (fused to EYFP) under the EF1a promoter, which can be deactivated 
            (deleted) by recombination between the flanking loxP sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  AUTHORS   McDevitt RA, Tiran-Cappello A, Shen H, Balderas I, Britt JP, Marino 
            RAM, Chung SL, Richie CT, Harvey BK, Bonci A
  TITLE     Serotonergic versus nonserotonergic dorsal raphe projection neurons:
            differential participation in reward circuitry.
  JOURNAL   Cell Rep. 2014 Sep 25;8(6):1857-1869. doi: 
            10.1016/j.celrep.2014.08.037. Epub 2014 Sep 18.
  PUBMED    25242321
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.08.037"; journalName: "Cell Rep"; date: 
            "2014-09-25- 25"; volume: "8"; issue: "6"; pages: "1857-1869"
COMMENT     Sequence Label: pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..50)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtgtcgtga ggtaccataa cttcgtatag catacattat acgaagttat gctagccacc
       61 atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct
      121 gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt
      181 gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca
      241 ggattcagca ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc
      301 tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt
      361 gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc
      421 tatgcagagt ggctgctcac ttgtcctgtc atccttatcc gcctga
//
