LOCUS       sequence-97135-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV packaging vector that expresses channel rhodopsin 2 (H134R) 
            (fused to EYFP) under the EF1a promoter, which can be deactivated 
            (deleted) by recombination between the flanking loxP sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   McDevitt RA, Tiran-Cappello A, Shen H, Balderas I, Britt JP, Marino 
            RAM, Chung SL, Richie CT, Harvey BK, Bonci A
  TITLE     Serotonergic versus nonserotonergic dorsal raphe projection neurons:
            differential participation in reward circuitry.
  JOURNAL   Cell Rep. 2014 Sep 25;8(6):1857-1869. doi: 
            10.1016/j.celrep.2014.08.037. Epub 2014 Sep 18.
  PUBMED    25242321
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.08.037"; journalName: "Cell Rep"; date: 
            "2014-09-25- 25"; volume: "8"; issue: "6"; pages: "1857-1869"
COMMENT     Sequence Label: pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..125
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      167..176
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
       61 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      121 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcggcc
      181 gctggcacgg ctccggcctc ggcttcgtct tcgacgagag tctcgacctc gatctccgtt
      241 ccgccgatgt tcagtttggt ggttttgcgg atatctccgt gaatcaatat gtgctcgtgg
      301 atcaggacgc gcaggtagtg tcccaacaac ccccaacaat ttttactcat cagatcaata
      361 atcgtgtgac ctacggtgga gccatagacg ctcaggacgc caaaaccttc gggccccaaa
      421 atgaagagaa ttgggaacat accccagctc acgaaaaaca gccatgccat gccggtcacg
      481 acctggcggc accgaccctt tggcacagta tgataaccct cgatatatgc tttggcggcg
      541 tgaaaaaatg tgttcgcgcc atagcacaat ccaagacaaa agaagatgac tttaacatag
      601 ccggttgcca tggcgctggt agccccccac acgatagtcc cgatgtctga gacaaggagt
      661 cccatggttc tcctgctgta gtcgttgctc aggccggtga ggttgctcag gcggataagg
      721 atgacaggac aagtgagcag ccactctgca tagcgcagcc actgcacccg gtgtcctgtg
      781 gcaaggtaga gcatagaggg attcttaaac tcaaaa
//
