LOCUS       sequence-97138-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4L1M
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggctctcat aagggatcat tgcaagaggt cataggttgg
      121 gggttaatag gatggaaata ctatgccaat gtgattggtc caatccagtg cgaaggcctg
      181 gccaacctgg gagtcacaca gattgcctgt gcagagaagc gtttcctgat tctgtcacgc
      241 aatggccgcg tgtacacaca ggcctataat agtgacacgc tggccccaca gctggtccaa
      301 ggccttgcct ccagaaacat tgtaaaaatt gctgcccatt ctgatggtca ccactaccta
      361 gccttggctg ctactggaga ggtgtactcc tggggctgtg gggacggcgg acggctgggc
      421 catggggaca ctgtgccttt ggaggagcct aaggtgatct ccgccttctc tggaaagcag
      481 gccgggaagc acgtggtgca catcgcttgc gggagcactt acagtgcggc catcactgcc
      541 gagggggagc tgtacacctg gggccgcggg aactacggcc ggctgggcca tggctccagt
      601 gaggacgagg ccattccgat gctggtagcc gggcttaaag gactgaaggt catcgatgtg
      661 gcgtgtggga gtggggatgc tcaaaccctg gctgtcactg agaacgggca agtgtggtct
      721 tggggagatg gtgactatgg g
//
