LOCUS       sequence-97140-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4L59
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgtcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggcaagaa agcgtggtgc tgggcatcct acctggaaga
      121 ggagaaagcg gtggcagtgc cggcgaagct gttcaaggag catcaatcct ttccatataa
      181 caaaaatgga ttcaaagttg gcatgaaatt agaaggcgtg gatcccgagc atcagtctgt
      241 gtactgtgtc ctcaccgtcg cggaggtttg tggataccgg ataaagcttc actttgatgg
      301 gtattctgat tgctatgact tctgggtgaa tgcagacgct ctggatatcc acccagttgg
      361 gtggtgtgag aaaaccggcc acaaactcca tcctccaaaa gggtataaag aagaagaatt
      421 caattggcag acctatctta agacatgtaa agctcaagct gctcctaagt cattatttga
      481 aaatcagaat ataacagtga tcccatcggg ctttcgagtt ggtatgaagc ttgaggcagt
      541 agacaaaaag aatccctcat tcatctgtgt tgctacggta acagatatgg tggacaatcg
      601 tttcctggta cattttgaca actgggatga gagctatgac tattggtgtg aagcatcaag
      661 tccacatatt catccagttg gttggtgtaa ggaacataga ag
//
