LOCUS       sequence-97144-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LDF
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(401..421)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(434..451)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             461..483
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    498..522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(522..541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(523..541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(625..644)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctcg agtgcggccg caagcttcgt catcatgatt
       61 cctgtatcgt ttgtagcatt ggccaaataa atgcctccaa ctggtttgca tacttcgtat
      121 aaattgtatt ttttgcattg taaaatctgc agttatcaaa cattctttta agctcgctgc
      181 taaactcaga taatgtctta tattctttat ttttagcttt acgcttcata gtttggatat
      241 cagtaggttc tttaataatc tcatagtagt ctgatgcttc ccctatgcta actggttttc
      301 taaacggcca tgcattttca tgccttgata gggtatcaag taactgccaa atttgatcgt
      361 tcatagagag gtcaacctta gattgctcga caggatcaat gccctggaaa tacaagtttt
      421 ctctgccgct gctgtgatga tgatgatgat gcatggtata tctccttctt aaagttaaac
      481 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt
      541 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
      601 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
      661 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
      721 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttg
//
