LOCUS       sequence-97145-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LDG
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..68
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaggagata taccatgcat catcatcatc atcacagcag cggcagagaa aacttgtatt
       61 tccagggctc aaacaaaaat caccaaaaac ttaaagaaaa taaagacatc ttctatgaac
      121 tgagtcaaaa acttattcag gacttgtata gtaaaattcc atactttgag agaaatggaa
      181 tcgaatattt tacagactcc aaatttttag atcttaaaca caaaaacaaa gaattttcaa
      241 ataattctga acttgaaatc aattttacac caaacaatga aattgagcag tctaatattg
      301 aaaaccaaat aactagaaga tctagcaaaa gagtctcttt tggtgctaat agcgaaatat
      361 caatcattga agcaacaata gatatgaact acaaagttat tcacctccca atatataatt
      421 attcagaaaa atggaagaag attatctaca aatattgtat cttaaatgaa attcactcat
      481 gcactttgat taaaaaagat gcatttaaag gaagatgtgt aatcgccgga tctcttatta
      541 gaaaagatga ctttgttctt gaatataaag gaaatctaat aactcagctt aacgaggcca
      601 aagagctgga agagaagtat gctcttagta atagggggtg ttatatgtat tacttcaaag
      661 ctaacgataa gaa
//
