LOCUS       sequence-97147-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     SGC Toronto Q1-Q3 2013
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4LEC
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tctagaatat tttgtttact ttaagaagga gatataccat gcatcatcat catcatcaca
       61 gcagcggcag agaaaacttg tatttccagg gcgagaccac ggaatttggg ttgcagaaat
      121 tccacaagcc tcttgcaact ttttcctttg caaaccacac gatccagatc cggcaggact
      181 ggagacacct gggagtcgca gcggtggttt gggatgcggc catcgttctt tccacatacc
      241 tggagatggg agctgtggag ctcaggggcc gctctgccgt ggagctgggt gctggcacgg
      301 ggctggtggg catagtggct gccctgctgg gtgctcatgt gactatcacg gatcgaaaag
      361 tagcattaga atttcttaaa tcaaacgttc aagccaactt acctcctcat atccaaacta
      421 aaactgttgt taaggagctg acttggggac aaaatttggg gagtttttct cctggagaat
      481 ttgacctgat acttggtgct gatatcatat atttagaaga aacattcaca gatcttcttc
      541 aaacactgga acatctctgt agcaatcact ctgtgattct tttagcatgc cgaattcgct
      601 atgaacggga taacaacttc ttagcaatgc tggagaggca atttattgtg agaaaggttc
      661 actacgatcc tgaaaaagat gtacatattt acgaagcaca gaagagaaac cagaaggagg
      721 acttatgatg acgaagcttg cggccgcact cgagcaccac caccaccaac cactgagatc
      781 cggctgctaa caaag
//
